Coding

Part:BBa_K4766010:Design

Designed by: Xinyu Wang   Group: iGEM23_DUT-China   (2023-10-05)


SpyTag-eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fluorescent protein mEGFP connected with SpyTag can be captured by proteins connected with SpyCatcher, playing a role as a signal molecule.

Source

https://www.fpbase.org/protein/megfp/

References

W. Kang, X. Ma, D. Kakarla, H. Zhang, Y. Fang, B. Chen, K. Zhu, D. Zheng, Z. Wu, B. Li, C. Xue, Angew. Chem. Int. Ed. 2022, 61, e202214001; Angew. Chem. 2022, 134, e202214001.

Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1).